Normal TROSY
●Large proteins
–Fast relaxation = Broader line widths
–Decrease in spectral resolution
1H – 15N TROSY
–Relaxation contributions from:
●Dipole-dipole (DD)
●Chemical shift anisotropy (CSA)
–DD and CSA interfere either constructively or destructively
–TROSY selects signals where destructive interference has occurred
●Cancelled out
–Results in sharper signals and increased resolution
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