●Large proteins –Fast relaxation = Broader line widths –Decrease in spectral resolution
1H – 15N TROSY –Relaxation contributions from:
●Dipole-dipole (DD)
●Chemical shift anisotropy (CSA) –DD and CSA interfere either constructively or destructively –TROSY selects signals where destructive interference has occurred
●Cancelled out –Results in sharper signals and increased resolution
